Vine maturity
‘Premier Russet’ has a late vine maturity and ‘Rio Grande Russet’ has a medium late maturity. 0–6.5) than ‘Premier Russet’ (6.0–7.5). For the progeny, mean trait values displayed a wide range of phenotypic variation (Additional file 4: Table S3), with a high correlation between years (> 0.70, P < 0.001) and across environments (> 0.55, P < 0.001). The broad-sense heritability for both years at each location was statistically significant, with H 2 estimates ranging from 0.83 to 0.93 (Table 2). These results were consistent for all vine maturity traits. When mean values for maturity were mapped to the linkage maps, a significant association was detected on chromosome 5 (Fig. 2, Additional file 6: Figure S2). This association was consistent between years and environments. Logarithm of the odds (LOD) scores ranged from of 21.2 and 34.1, well above the permutation thresholds. Collectively, they explained between 38 and 54% of the trait variance (Table 3). The peak of the LOD profile was consistently detected at 15.0 cM (Table 3, Fig. 2). The best simple model for maturity was a double-simplex configuration (AAAB ? AAAB) on homologous chromosomes H2 and H8 of Rio Grande russet (RGR) and Premier Russet (PR), respectively (Additional file 6: Figure S2). This model implies a QTL with allele effects from both parents, and with the B allele associated with earlier maturity (P < 0.001). Thus, progeny with two copies of the B allele (AABB) had on average earlier maturity (3.1–4.0) than progeny with a single copy (AAAB, 4.5–6.2) or lacking the B allele (AAAA, 6.4–7.5).
LOD score shipments out of QTLs for qualities at readiness locus region on chromosome 5. For each committee about contour signifies an alternate trait. LOD tolerance 95% rely on intervals is actually listed in Desk step three. Ca, citric acid 2010; EB, early blight; FBE, bud-prevent fry colour Idaho; GH, progress habit; GLU, glucose; M95, vine readiness at 95DAP; M120, vine readiness during the 120DAP; TSHA, tuber figure; VW, Verticillium wilt; ID, Idaho; NC, North carolina; DAP, weeks after growing; wide variety 10 and you can eleven about characteristic brands indicate many years 2010 and you can 2011, correspondingly
The SNP nearest toward QTL for readiness that had an excellent double-simplex configuration try solcap_snp_c2_11605, located at nine.step one cM. Regression of your own readiness mean opinions to the genotype of SNP explained ranging from 38 and you will fifty% of phenotypic variance. The latest height of the LOD bend (15 cM) is arranged within a gap ranging from markers one spanned 8 so you’re able to 10 cM, with the solcap_snp_c2_11605 located on that section of the gap. This place ports the fresh Solanum tuberosum bicycling DOF foundation gene 1 (StCDF1, PGSC0003DMG400018408), which was considered a major regulator out-of potato bush maturity . Visual image of one’s hereditary and you may real (Mb) point from homologous chromosomes H2 and you will H8 showed the fresh asked contour profile and you will complement better having chromosome construction exhibiting a high knowledge off concordance between your genetic standing (cM) and you can real venue (Mb) (Even more document 7: Shape S3).
Several qualities co-localize from the readiness locus area to the chromosome 5
Seven traits including citric acidic (CANC10), progress practice (GHID, GHNC), bud-stop fry color (FBEID), early blight rating (EBID11), tuber sugar (GLUID11), tuber shape (TSHAID), and you may Verticillium wilt get (VWID11) co-localized having vine maturity attributes (MAT95ID, MAT95NC, MAT120ID, MAT120NC) towards the chromosome 5 (Desk 3, Fig. 2). An educated hereditary model for all characteristics, but GLUID11 and you can FBEID try a two fold-simplex impact on H2 and you can H8 towards SNP, solcap_snp_c2_11605, having this arrangement (Desk step 3). If the residuals regarding escort review Chico regression of all the half a dozen traits into the vine readiness was basically analyzed, zero extreme aftereffect of a good QTL try receive to own EBID11, TSHAID, and you can GHNC. To own growth behavior (GHID) and you will VWID11, a serious QTL was noticed only if corrected for maturity during the 95DAP but was not significant from the 120DAP. Research off sugar density (GLUID11) presented a LOD off step three.9 in the 21 cM, that have an excellent QTL affect H2 and you may H5. Once modifying getting readiness, a top on LOD curve getting GLUID11 is observed only below the 95% permutation tolerance.