For the chromosome 4, all about three QTLs was indeed from the 66
Package plots demonstrating the consequences of your marker genotypic categories (AAAA, AAAB, AABB, ABBB, BBBB) out of 4 applicant SNPs towards the tuber glucose focus (GLU); bud-prevent fry colour (FBE); stem-tuber fry colour (FSE); ID, Idaho
The newest co-localization out-of several QTLs from the maturity locus part within research try consistent with the observed habits from phenotypic correlations. 01) with many attributes overlapping an equivalent hereditary standing (Fig. 1). As an example, EBID11 and VWID11 score both showed a powerful negative correlation with MAT95ID and you will MAT120ID. So it development can get give an explanation for big impact QTL having after maturity directly with the put off onset of VW and you will EB, or alternatively, very early maturity causing very early start of VW and you may EB. A relatively highest relationship was also noticed anywhere between vine maturity and progress behavior, that have very early maturity from the significantly more dispersed growth behavior. In contrast, a minimal relationship (roentgen ? 0.2) anywhere between vine readiness and you will one another tuber glucose quantity and you will bud-prevent fry color is actually consistent with the latest models of towards QTL outcomes.
Tuber sugar, bud-avoid and you can stalk-avoid fry color
Together with Top- und Single-Dating-Seite chromosome 5, significant QTLs having sugar, bud-stop and you may stem-end fry colour was indeed detected inside overlapping regions of chromosomes cuatro, six, 10, and you can 11. 0 cM on the LOD score between 4.5 to 6.0 (Dining table step three, Fig. 4). These QTLs told me ranging from eight.2 and you will 11.3% of your own attribute variance. Analysis of different effortless models expressed an individual better-suitable design for everyone three traits, which have a double-simplex allele (AAAB ? AAAB) toward homologous chromosomes H2 and you will H5 away from RGR and you will Public relations, respectively (Dining table step three, More document 8: Contour S4). To own sugar, the fresh new B allele try regarding the high glucose attention, when you find yourself to have bud-avoid and you can stem-stop fry color, the existence of the latest B allele expressed straight down reflectance viewpoints (Fig. 3b).
LOD score shipment regarding QTLs for three traits along side twelve potato chromosomes (Chr). Abbreviations try like in Fig. step one
A candidate SNP for a double-simplex configuration on H2 and H5 is solcap_c2_12976 at position 67.4 cM. On the reference PGSC v4.03 Pseudomolecules, this SNP maps to the long arm of chromosome 4 at position 69.1 Mb on the F-box and WD40 domain protein gene (PGSC0003DMG400003339), which is close (< 2 Mb) to several genes associated with starch/sugar inter-conversion as annotated in the potato reference genome, and/or associated with tuber quality traits . They include alpha amylase (AMY-4/1 PGSC0003DMG400007974, AMY-4/2 PGSC0003DMG400009891), hexose transporter (HT-4/3, PGSC0003DMG400009994), invertase (NV-4, PGSC0003DMG400009936), and starch branching enzyme I (SBE I, PGSC0003DMG400009981).
On chromosome 6, significant QTLs mapped at 53 cM for glucose and at 43 cM for FBE and SBE, respectively. The LOD profiles ranged from 5.4 to 7.6 and explained between 10.8 and 16.1% of the trait variance, respectively (Table 3, Additional file 9: Figure S5). The best model for all three traits was a double-simplex configuration with allele effects on H2 and H5. As in chromosome 4, the presence of the B alleles was associated with an increase in glucose concentration and a decrease in reflectance readings (Fig. 3c). There are four candidate SNPs with a double simplex configuration nearby (solcap_snp_c2_33777, solcap_snp_c1_10130, solcap_snp_c2_5769, solcap_snp_c2_56145, c1_3003). All were significantly (P-value < 0.0001) associated with the mean trait values, however, solcap_snp_c2_56145 had on average the highest percent of variance explained (9% to 16%). The SNP solcap_snp_c2_56145 maps at position 51.4 Mb of the potato reference genome on the ATP binding protein gene PGSC0003DMG400033089. This SNP is located in a region (
seven Mb) that contains multiple genetics regarding the starch/sugar inter-conversion process in addition to hexokinase (HXK-6, PGSC0003DMG400016521), fructokinase (FRK-6/1, PGSC0003DMG400027017, FRK-6/2, PGSC0003DMG400026916), and you may invertase (INV-na-6/1, PGSC0003DMG400026107, INV-6/dos, PGSC0003DMG400033142) genes.